Journal: Cell Death & Disease

Article Title: PUM1 knockdown prevents tumor progression by activating the PERK/eIF2/ATF4 signaling pathway in pancreatic adenocarcinoma cells

doi: 10.1038/s41419-019-1839-z

Figure Lengend Snippet: a PUM1 mRNA expression levels in PAAD tissues (T) and normal tissues (N) analyzed by gene expression profiling interactive analysis (GEPIA). * P < 0.05. b Analysis of the relationship between PUM1 mRNA expression and overall survival of PAAD patients analyzed using The Cancer Genome Atlas (TCGA)

Article Snippet: The PUM1 primary antibody was purchased from ABclonal Technology (Wuhan, China).

Techniques: Expressing, Gene Expression

Journal: Cell Death & Disease

Article Title: PUM1 knockdown prevents tumor progression by activating the PERK/eIF2/ATF4 signaling pathway in pancreatic adenocarcinoma cells

doi: 10.1038/s41419-019-1839-z

Figure Lengend Snippet: a Representative images of PUM1 expression by immunohistochemistry (IHC) analysis in tissue microarrays representing 60 PDAC patients. b Comparison of total scores for PUM1 assessment on tissue microarrays slides. Total score = positive staining scores × staining intensity score. * P < 0.05. c The overall survival rate of patients with PDAC was evaluated based on PUM1 expression using Kaplan–Meier analysis ( n = 60, P = 0.022)

Article Snippet: The PUM1 primary antibody was purchased from ABclonal Technology (Wuhan, China).

Techniques: Expressing, Immunohistochemistry, Comparison, Staining

Journal: Cell Death & Disease

Article Title: PUM1 knockdown prevents tumor progression by activating the PERK/eIF2/ATF4 signaling pathway in pancreatic adenocarcinoma cells

doi: 10.1038/s41419-019-1839-z

Figure Lengend Snippet: The relationship between PUM1 protein levels and clinic characteristics of patients with pancreatic adenocarcinoma

Article Snippet: The PUM1 primary antibody was purchased from ABclonal Technology (Wuhan, China).

Techniques: Expressing

Journal: Cell Death & Disease

Article Title: PUM1 knockdown prevents tumor progression by activating the PERK/eIF2/ATF4 signaling pathway in pancreatic adenocarcinoma cells

doi: 10.1038/s41419-019-1839-z

Figure Lengend Snippet: PUM1 mRNA ( a ) and protein ( b ) expression levels in PDAC cell lines (BxPC-3, AsPC-1, MIA PaCa-2, and PANC-1) and human pancreatic duct epithelial cells HPDE6-C7. c PUM1 expression levels in cells infected with lentivirus expressing the shRNA of PUM1 (shPUM1) or negative (NC) control empty lentivirus. d , e Effect of PUM1 knockdown on the OD values at 490 nm. f Effect of PUM1 knockdown on early apoptosis rate. b , c , f The representative graphs are on the left, and the statistical results are on the right. n = 3, * P < 0.05, for a – f

Article Snippet: The PUM1 primary antibody was purchased from ABclonal Technology (Wuhan, China).

Techniques: Expressing, Infection, shRNA, Control, Knockdown

Journal: Cell Death & Disease

Article Title: PUM1 knockdown prevents tumor progression by activating the PERK/eIF2/ATF4 signaling pathway in pancreatic adenocarcinoma cells

doi: 10.1038/s41419-019-1839-z

Figure Lengend Snippet: Effect of PUM1 knockdown on cell migration ( a ) and invasion ( b ) detected by Transwell assays. Representative graphs (left). Bar graph showing the statistical result of migratory or invasive cells (right). c Effect of PUM1 knockdown on the expression of MMP9, VEGF, vimentin, and E-cadherin. Representative graphs of western blotting (left). Relative protein expression in reference to GAPDH (right). n = 3, * P < 0.05, for a – c

Article Snippet: The PUM1 primary antibody was purchased from ABclonal Technology (Wuhan, China).

Techniques: Knockdown, Migration, Expressing, Western Blot

Journal: Cell Death & Disease

Article Title: PUM1 knockdown prevents tumor progression by activating the PERK/eIF2/ATF4 signaling pathway in pancreatic adenocarcinoma cells

doi: 10.1038/s41419-019-1839-z

Figure Lengend Snippet: a PUM1 expression levels in tumors formed by the shPUM1 or NC group cells. b , c Effect of PUM1 knockdown on the growth of subcutaneous xenograft tumor. b Pictures of subcutaneous xenograft tumor. c Tumor growth curve. n = 8, * P < 0.05. d , e Effect of PUM1 knockdown on lung metastases examined by hematoxylin and eosin staining, or Ki67 expression detected by immunohistochemistry in the lung tissues of a lung metastasis mouse model

Article Snippet: The PUM1 primary antibody was purchased from ABclonal Technology (Wuhan, China).

Techniques: Expressing, Knockdown, Staining, Immunohistochemistry

Journal: Cell Death & Disease

Article Title: PUM1 knockdown prevents tumor progression by activating the PERK/eIF2/ATF4 signaling pathway in pancreatic adenocarcinoma cells

doi: 10.1038/s41419-019-1839-z

Figure Lengend Snippet: Volcano plot ( a ), scatter plot ( b ), and cluster gram ( c ) of the cDNA microarrays. d Effect of PUM1 knockdown on the expression of key components of the eIF2 signaling pathway. Left: Representative graphs of western blotting. Right: Statistical result of the relative protein expression in reference to GAPDH. n = 3, * P < 0.05

Article Snippet: The PUM1 primary antibody was purchased from ABclonal Technology (Wuhan, China).

Techniques: Knockdown, Expressing, Western Blot

Journal: Cell Death & Disease

Article Title: PUM1 knockdown prevents tumor progression by activating the PERK/eIF2/ATF4 signaling pathway in pancreatic adenocarcinoma cells

doi: 10.1038/s41419-019-1839-z

Figure Lengend Snippet: a Representative pictures showing p-PERK levels in 60 PDAC tissues on tissue microarray examined by immunohistochemical analysis. b Linear regression analysis showing the correlation of PUM1 and p-PERK levels in PDAC tissues ( n = 60)

Article Snippet: The PUM1 primary antibody was purchased from ABclonal Technology (Wuhan, China).

Techniques: Microarray, Immunohistochemical staining